An optimized negative-staining protocol of electron microscopy for apoE4•POPC lipoprotein
نویسندگان
چکیده
منابع مشابه
An optimized negative-staining protocol of electron microscopy for apoE4•POPC lipoprotein
Apolipoprotein E (apoE), one of the major protein components of lipoproteins in the peripheral and central nervous systems, regulates cholesterol metabolism through its interaction with members of the low density lipoprotein receptor family. One key to understanding apoE function is determining the structure of lipid-bound forms of apoE. Negative-staining (NS) electron microscopy (EM) is an eas...
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Structural determination of proteins is rather challenging for proteins with molecular masses between 40 - 200 kDa. Considering that more than half of natural proteins have a molecular mass between 40 - 200 kDa, a robust and high-throughput method with a nanometer resolution capability is needed. Negative staining (NS) electron microscopy (EM) is an easy, rapid, and qualitative approach which h...
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ژورنال
عنوان ژورنال: Journal of Lipid Research
سال: 2010
ISSN: 0022-2275
DOI: 10.1194/jlr.d002493